Composite

Part:BBa_K2637041:Design

Designed by: Jinyu Yang   Group: iGEM18_Tianjin   (2018-10-05)


Gal2 promoter+NanoLuc+ADH1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 529


Design Notes

No.


Source

We copied the Gal2 promoter and ADH1T terminator sequences from plasmid BY4741 and utilized the part numbered BBa_K1680009 to obtain the sequence of NanoLuc.

References